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The Proteome of Native Adult Müller Glial Cells From Murine Retina

Grosche, Antje ; Hauser, Alexandra ; Lepper, Marlen Franziska ; Mayo, Rebecca ; von Toerne, Christine ; Merl-Pham, Juliane ; Hauck, Stefanie M.



Abstract

To date, the proteomic profiling of Muller cells, the dominant macroglia of the retina, has been hampered because of the absence of suitable enrichment methods. We established a novel protocol to isolate native, intact Muller cells from adult murine retinae at excellent purity which retain in situ morphology and are well suited for proteomic analyses. Two different strategies of sample ...

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