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Pitfalls in assessing microvascular endothelial barrier function: impedance-based devices versus the classic macromolecular tracer assay

Bischoff, Iris ; Hornburger, Michael C. ; Mayer, Bettina A. ; Beyerle, Andrea ; Wegener, Joachim ; Fürst, Robert


The most frequently used parameters to describe the barrier properties of endothelial cells (ECs) in vitro are (i) the macromolecular permeability, indicating the flux of a macromolecular tracer across the endothelium, and (ii) electrical impedance of ECs grown on gold-film electrodes reporting on the cell layer's tightness for ion flow. Due to the experimental differences between these ...


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