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Super-resolution in situ analysis of active ribosomal DNA chromatin organization in the nucleolus

Maiser, Andreas ; Dillinger, Stefan ; Längst, Gernot ; Schermelleh, Lothar ; Leonhardt, Heinrich ; Németh, Attila
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Date of publication of this fulltext: 13 May 2020 10:15


Abstract

Ribosomal RNA (rRNA) transcription by RNA polymerase I (Pol I) is the first key step of ribosome biogenesis. While the molecular mechanisms of rRNA transcription regulation have been elucidated in great detail, the functional organization of the multicopy rRNA gene clusters (rDNA) in the nucleolus is less well understood. Here we apply super-resolution 3D structured illumination microscopy ...

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