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Optimized Protocol for the In Situ Derivatization of Glutathione with N-Ethylmaleimide in Cultured Cells and the Simultaneous Determination of Glutathione/Glutathione Disulfide Ratio by HPLC-UV-QTOF-MS

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Sun, Xueni ; Berger, Raffaela ; Heinrich, Paul ; Marchiq, Ibtissam ; Pouyssegur, Jacques ; Renner, Kathrin ; Oefner, Peter J. ; Dettmer, Katja
License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 30 Jul 2020 06:40


Glutathione (GSH) and glutathione disulfide (GSSG) are commonly used to assess the oxidative status of a biological system. Various protocols are available for the analysis of GSH and GSSG in biomedical specimens. In this study, we present an optimized protocol for the in situ derivatization of GSH withN-ethylmaleimide (NEM) to prevent GSH autooxidation, and thus to preserve the GSH/GSSG ratio ...


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