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Complete sequential assignment and secondary structure prediction of the cannulae forming protein CanA from the hyperthermophilic archaeon Pyrodictium abyssi

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Kalbitzer, Hans Robert ; Kreitner, Raphael ; Munte, Claudia E. ; Singer, Katrin ; Stetter, Karl Otto ; Horn, Gudrun ; Kremer, Werner
License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 05 Feb 2021 08:53


CanA from Pyrodictium abyssi forms a heat-resistant organic hollow-fiber network together with CanB and CanC. An N-terminally truncated construct of CanA (K1-CanA) gave NMR spectra of good quality that could be assigned by threedimensional NMR methods on 15N and 13C–15N enriched protein. We assigned the chemical shifts of 96% of all backbone 1HN atoms, 98% of all backbone 15N atoms, 100% of all ...


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