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NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells

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Grätz, Lukas ; Tropmann, Katharina ; Bresinsky, Merlin ; Müller, Christoph ; Bernhardt, Günther ; Pockes, Steffen
License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 19 Feb 2021 13:06


Fluorescence/luminescence-based techniques play an increasingly important role in the development of test systems for the characterization of future drug candidates, especially in terms of receptor binding in the field of G protein-coupled receptors (GPCRs). In this article, we present the establishment of a homogeneous live cell-based BRET binding assay for the histamine H-2 receptor with ...


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