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NanoBRET binding assay for histamine H2 receptor ligands using live recombinant HEK293T cells

URN to cite this document:
urn:nbn:de:bvb:355-epub-449913
DOI to cite this document:
10.5283/epub.44991
Grätz, Lukas ; Tropmann, Katharina ; Bresinsky, Merlin ; Müller, Christoph ; Bernhardt, Günther ; Pockes, Steffen
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Date of publication of this fulltext: 19 Feb 2021 13:06



Abstract

Fluorescence/luminescence-based techniques play an increasingly important role in the development of test systems for the characterization of future drug candidates, especially in terms of receptor binding in the field of G protein-coupled receptors (GPCRs). In this article, we present the establishment of a homogeneous live cell-based BRET binding assay for the histamine H-2 receptor with ...

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