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Electron microscopy of Drosophila garland cell nephrocytes: Optimal preparation, immunostaining and STEM tomography

Hochapfel, Florian ; Denk, Lucia ; Maaßen, Christine ; Zaytseva, Yulia ; Rachel, Reinhard ; Witzgall, Ralph ; Krahn, Michael P.


Due to its structural and molecular similarities to mammalian podocytes, the Drosophila nephrocyte emerged as a model system to study podocyte development and associated diseases. Similar to podocytes, nephrocytes establish a slit diaphragm between foot process-like structures in order to filter the hemolymph. One major obstacle in nephrocyte research is the distinct visualization of this ...


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