Jiang, Jiebing ; Sun, Xueni ; She, Xiaojian ; Li, Jiajia ; Li, Yan ; Deng, Chunhui ; Duan, Gengli
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Microchimica Acta |
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| Verlag: | SPRINGER WIEN |
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| Ort der Veröffentlichung: | WIEN |
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| Band: | 185 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 6 |
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| Datum: | 2018 |
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| Institutionen: | Medizin > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1007/s00604-018-2837-z | DOI |
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| Stichwörter / Keywords: | ION AFFINITY-CHROMATOGRAPHY; HIGHLY SELECTIVE ENRICHMENT; METAL-ORGANIC FRAMEWORK; PHOSPHORYLATED PEPTIDES; PROTEIN-PHOSPHORYLATION; MASS-SPECTROMETRY; PHOSPHOPROTEOME RESEARCH; MALDI; GRAPHENE; NANOPARTICLES; Metal ions combination; Immobilized metal ion affinity chromatography; MALDI-TOF mass spectrometry; Polydopamine; Affinity probe |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 47150 |
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Web of Science
Zusammenfassung
Magnetic microspheres (Fe3O4) were coated with polydopamine (PDA) and loaded with the metal ions Ti(IV) and Nb(V) to give a material of type Fe3O4@PDA-Ti/Nb. It is shown to be useful for affinity chromatography and for enrichment of phosphopeptides from both standard protein solutions and real samples. For comparison, such microspheres loaded with single metal ions only (Fe3O4@PDA-Ti and ...
Zusammenfassung
Magnetic microspheres (Fe3O4) were coated with polydopamine (PDA) and loaded with the metal ions Ti(IV) and Nb(V) to give a material of type Fe3O4@PDA-Ti/Nb. It is shown to be useful for affinity chromatography and for enrichment of phosphopeptides from both standard protein solutions and real samples. For comparison, such microspheres loaded with single metal ions only (Fe3O4@PDA-Ti and Fe3O4@PDA-Nb) and their physical mixtures were also investigated under identical conditions. The binary metal ion-loaded magnetic microspheres display better enrichment efficiency than the single metal ion-loaded microspheres and their physical mixture. Both multiphosphopeptides and monophosphopeptides can be extracted. The Fe3O4@PDA-Ti/Nb microspheres exhibit ultra-high sensitivity (the lowest detection amount being 2 fmol) and selectivity at a low mass ratio such as in case of beta-casein/BSA (1:1000).
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