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Magnetic microspheres modified with Ti(IV) and Nb(V) for enrichment of phosphopeptides

Jiang, Jiebing ; Sun, Xueni ; She, Xiaojian ; Li, Jiajia ; Li, Yan ; Deng, Chunhui ; Duan, Gengli


Magnetic microspheres (Fe3O4) were coated with polydopamine (PDA) and loaded with the metal ions Ti(IV) and Nb(V) to give a material of type Fe3O4@PDA-Ti/Nb. It is shown to be useful for affinity chromatography and for enrichment of phosphopeptides from both standard protein solutions and real samples. For comparison, such microspheres loaded with single metal ions only (Fe3O4@PDA-Ti and ...


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