Abstract
Today, the search for therapeutic options to treat retinal degeneration often relies on an in-depth understanding of the underlying pathological events. Alternatively, it is conceivable to search, in an undirected screening approach, for chemical compounds affecting disease outcome. For both approaches, there is an urgent need for in vitro and, ideally, in vivo disease models that adequately ...
Abstract
Today, the search for therapeutic options to treat retinal degeneration often relies on an in-depth understanding of the underlying pathological events. Alternatively, it is conceivable to search, in an undirected screening approach, for chemical compounds affecting disease outcome. For both approaches, there is an urgent need for in vitro and, ideally, in vivo disease models that adequately reflect the site of pathology. Currently available animal models possess limitations as they often develop only defined aspects of disease. Primary cell cultures, derived from the posterior pole of the eye, can only be obtained after invasive surgery or are available post mortem, but due to rapid cell senescence are not suited for longterm analysis. Immortalized retinal cell lines, on the other hand, differ in many aspects from native cells. In this situation, a promising alternative could arise from induced pluripotent stem cells (iPSCs). This cell species can be generated via non-invasive techniques, they are patient-specific, can be propagated indefinitely, and theoretically can be differentiated in all types of retinal cells due to their pluripotent capacities. Importantly, the iPSC-derived retinal cells greatly resemble native cells in many characteristic traits. In this review we present a selection of established in vivo und in vitro models for retinal degenerative disease. We also discuss the potential of iPSCs for personalized in vitro modelling and provide an overview of existent iPSC-derived cell types of the posterior pole, particularly for cells of the retinal pigment epithelium. We finally give an outlook for the potential of such cells for basic research in ophthalmology.
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