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Cellular Stoichiometry of Methyl-Accepting Chemotaxis Proteins in Sinorhizobium meliloti

Zatakia, Hardik M. ; Arapov, Timofey D. ; Meier, Veronika M. ; Scharf, Birgit E. ; Zhulin, Igor B.



Abstract

The chemosensory system in Sinorhizobium meliloti has several important deviations from the widely studied enterobacterial paradigm. To better understand the differences between the two systems and how they are optimally tuned, we determined the cellular stoichiometry of the methyl-accepting chemotaxis proteins (MCPs) and the histidine kinase CheA in S. meliloti. Quantitative immunoblotting was ...

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