Standardized kinetic microassay to quantify differential chemosensitivity on the basis of proliferative activity

URN to cite this document:

urn:nbn:de:bvb:355-epub-48190 Copy

DOI to cite this document:

10.5283/epub.4819 Copy

Bernhardt, Günther ; Reile, Herta ; Birnböck, Herbert ; Spruss, Thilo ; Schönenberger, Helmut

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Date of publication of this fulltext: 05 Aug 2009 13:48

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Details

Item type:	Article
Journal or Publication Title:	Journal of cancer research and clinical oncology
Volume:	118
Number of Issue or Book Chapter:	1
Page Range:	pp. 35-43
Date:	1992
Institutions:	Chemistry and Pharmacy > Institute of Pharmacy > Alumni or Retired Professors > Prof. Schönenberger Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Projects:	SFB 234
Identification Number:	Value Type 1309532 PubMed ID
Classification:	Notation Type Adenocarcinoma/drug therapy MESH Antineoplastic Agents/pharmacology MESH Breast Neoplasms/drug therapy MESH Carcinoma, Intraductal, Noninfiltrating/drug therapy MESH Cell Division/drug effects MESH Cisplatin/pharmacology MESH Diethylstilbestrol/pharmacology MESH Drug Screening Assays, Antitumor/methods MESH Female MESH Fluorouracil/pharmacology MESH Gentian Violet MESH Humans MESH Kinetics MESH Melphalan/pharmacology MESH Neoplasms, Hormone-Dependent/drug therapy MESH Receptors, Estrogen/analysis MESH Receptors, Progesterone/analysis MESH Tumor Cells, Cultured/drug effects MESH Vinblastine/pharmacology MESH
Dewey Decimal Classification:	500 Science > 570 Life sciences 500 Science > 540 Chemistry & allied sciences
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	4819

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Abstract

Conventionally in vitro cytotoxicity assays are performed as single-end-point determinations. To compensate for the diversity of growth rates among different cell lines in this report we describe a computerized kinetic chemosensitivity assay based on quantification of biomass by staining cells with crystal violet. As a prerequisite four human breast cancer cell lines (MDA-MB-231, MCF-7, T-47-D ...

Abstract

Conventionally in vitro cytotoxicity assays are performed as single-end-point determinations. To compensate for the diversity of growth rates among different cell lines in this report we describe a computerized kinetic chemosensitivity assay based on quantification of biomass by staining cells with crystal violet. As a prerequisite four human breast cancer cell lines (MDA-MB-231, MCF-7, T-47-D and ZR-75-1) were characterized with regard to oestrogen and progesterone receptor content, modal chromosome number and proliferation kinetics depending on the number of passages in culture. With prolonged time in culture for ZR-75-1 exposed to various concentrations of cisplatinum a dose-related increase in drug effect was observed. Owing to a correction of the T/C values for the initial cell mass (at the time when drug is added) a sharp distinction between cytostatic and cytocidal drug effects becomes obvious in plots of corrected T/C values versus time of incubation. The influence of the untreated control on the corrected T/C values and possible time courses of theoretical inhibition profiles (reflecting cytostatic, transient cytotoxic or cytocidal drug effects as well as development of resistance) and their relationship to the corresponding growth curves of drug-treated cells are discussed. Chemosensitivity assays with diethylstilbestrol dipropionate, tamoxifen, melphalan, cisplatinum, vinblastine, Adriamycin and 5-fluorouracil prove the theoretical considerations to be true for MDA-MB-231, MCF-7, T-47-D and ZR-75-1 human breast cancer cell lines in practice.

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