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Standardized kinetic microassay to quantify differential chemosensitivity on the basis of proliferative activity

URN to cite this document:
urn:nbn:de:bvb:355-epub-48190
Bernhardt, Günther ; Reile, Herta ; Birnböck, Herbert ; Spruss, Thilo ; Schönenberger, Helmut
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Date of publication of this fulltext: 05 Aug 2009 13:48


Abstract

Conventionally in vitro cytotoxicity assays are performed as single-end-point determinations. To compensate for the diversity of growth rates among different cell lines in this report we describe a computerized kinetic chemosensitivity assay based on quantification of biomass by staining cells with crystal violet. As a prerequisite four human breast cancer cell lines (MDA-MB-231, MCF-7, T-47-D ...

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