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Generation of a Stand‐Alone Tryptophan Synthase α‐Subunit by Mimicking an Evolutionary Blueprint

Schupfner, Michael ; Busch, Florian ; Wysocki, Vicki H. ; Sterner, Reinhard



Abstract

The alpha beta beta alpha tryptophan synthase (TS), which is part of primary metabolism, is a paradigm for allosteric communication in multienzyme complexes. In particular, the intrinsically low catalytic activity of the alpha-subunit TrpA is stimulated several hundredfold through the interaction with the beta-subunit TrpB1. The BX1 protein from Zea mays (zmBX1), which is part of secondary ...

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