Dokumentenart: | Artikel | ||||
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Titel eines Journals oder einer Zeitschrift: | Nucleic Acids Research | ||||
Verlag: | OXFORD UNIV PRESS | ||||
Ort der Veröffentlichung: | OXFORD | ||||
Band: | 47 | ||||
Nummer des Zeitschriftenheftes oder des Kapitels: | 10 | ||||
Seitenbereich: | S. 5016-5037 | ||||
Datum: | 2019 | ||||
Institutionen: | Biologie und Vorklinische Medizin > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann | ||||
Identifikationsnummer: |
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Stichwörter / Keywords: | DENSITY-DEPENDENT APOPTOSIS; H4K16 ACETYLATION; ACETYLTRANSFERASE HMOF; NEUTROPHIL APOPTOSIS; HEMATOPOIETIC STEM; DIMETHYL-SULFOXIDE; TOPOISOMERASE-I; GASTRIC-CANCER; MALES ABSENT; NG2 ANTIGEN; | ||||
Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie | ||||
Status: | Veröffentlicht | ||||
Begutachtet: | Ja, diese Version wurde begutachtet | ||||
An der Universität Regensburg entstanden: | Ja | ||||
Dokumenten-ID: | 48570 |
Zusammenfassung
Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally ...
Zusammenfassung
Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death.
Metadaten zuletzt geändert: 10 Feb 2022 08:38