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Determination of enrichment factors for modified RNA in MeRIP experiments

Slama, Kaouthar ; Galliot, Aurellia ; Weichmann, Franziska ; Hertler, Jasmin ; Feederle, Regina ; Meister, Gunter ; Helm, Mark


In the growing field of RNA modification, precipitation techniques using antibodies play an important role. However, little is known about their specificities and protocols are missing to assess their effectiveness. Here we present a method to assess enrichment factors after MeRIP-type pulldown experiments, here exemplified with a commercial antibody against N6-methyladenosine (m(6)A). Testing ...


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