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Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

Urdaneta, Erika C. ; Vieira-Vieira, Carlos H. ; Hick, Timon ; Wessels, Hans-Herrmann ; Figini, Davide ; Moschall, Rebecca ; Medenbach, Jan ; Ohler, Uwe ; Granneman, Sander ; Selbach, Matthias ; Beckmann, Benedikt M.



Abstract

Recent methodological advances allowed the identification of an increasing number of RNA-binding proteins (RBPs) and their RNA-binding sites. Most of those methods rely, however, on capturing proteins associated to polyadenylated RNAs which neglects RBPs bound to non-adenylate RNA classes (tRNA, rRNA, pre-mRNA) as well as the vast majority of species that lack poly-A tails in their mRNAs ...

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