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Human platelet lysate as validated replacement for animal serum to assess chemosensitivity

Pons, Miriam ; ; ; ; ; ; ; ; ; ;


Experiments with cultured mammalian cells represent an in vitro alternative to animal experiments. Fetal calf serum (FCS) is the most commonly used medium supplement worldwide. FCS contains a variable mixture of growth factors and cytokines that support cell proliferation. This undefined nature of FCS is a source of experimental variation, undesired immune responses, possible contaminations and, ...


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