Zusammenfassung
In this report, the effects of forced convection on scanning electrochemical microscopy (SECM) studies of enzymes in the context of the generator-collector mode (G/C mode) were investigated. Forced convection was generated via an electrical high precision stirrer integrated into the electrochemical cell. Circular spots of glucose oxidase were immobilized on a gold support serving as model ...
Zusammenfassung
In this report, the effects of forced convection on scanning electrochemical microscopy (SECM) studies of enzymes in the context of the generator-collector mode (G/C mode) were investigated. Forced convection was generated via an electrical high precision stirrer integrated into the electrochemical cell. Circular spots of glucose oxidase were immobilized on a gold support serving as model substrate. The diffusion layer of enzymatically generated H2O2 was characterized recording probe scan curves (PSCs) in z-direction. Furthermore, the enzyme-modified surfaces were investigated via constant-height SECM imaging in feedback mode and in G/C mode. For methodical comparison all sets of experiments were performed in quiescent solution (conventional approach) and with forced convection, respectively. In contrast to a growing diffusion layer without forced convection by applying forced convection, a constant diffusion layer of produced H2O2 was observed. Hence, via hydrodynamic SECM time-independent images within a reasonable time scale of SECM measurements in G/C mode were enabled and their resolution was enhanced.
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