Purpose: Recent studies demonstrated a contribution of adrenoceptors (ARs) to osteoarthritis (OA) pathogenesis. Several AR subtypes are expressed in joint tissues and the beta 2-AR subtype seems to play a major role during OA progression. However, the importance of beta 2-AR has not yet been investigated in knee OA. Therefore, we examined the development of knee OA in beta 2-AR-deficient (Adrb2(-/-) ) mice after surgical OA induction. Methods: OA was induced by destabilization of the medial meniscus (DMM) in male wildtype (WT) and Adrb2(-/-) mice. Cartilage degeneration and synovial inflammation were evaluated by histological scoring. Subchondral bone remodeling was analyzed using micro-CT. Osteoblast (alkaline phosphatase - ALP) and osteoclast (cathepsin K - CatK) activity were analyzed by immunostainings. To evaluate beta 2-AR deficiency-associated effects, body weight, sympathetic tone (splenic norepinephrine (NE) via HPLC) and serum leptin levels (ELISA) were determined. Expression of the second major AR, the alpha 2-AR, was analyzed in joint tissues by immunostaining. Results: WT and Adrb2(-/-) DMM mice developed comparable changes in cartilage degeneration and synovial inflammation. Adrb2(-/-) DMM mice displayed elevated calcified cartilage and subchondral bone plate thickness as well as increased epiphyseal BV/TV compared to WTs, while there were no significant differences in Sham animals. In the subchondral bone of Adrb2(-/-) mice, osteoblasts activity increased and osteoclast activity deceased. Adrb2(-/-) mice had significantly higher body weight and fat mass compared to WT mice. Serum leptin levels increased in Adrb2(-/-) DMM compared to WT DMM without any difference between the respective Shams. There was no difference in the development of meniscal ossicles and osteophytes or in the subarticular trabecular microstructure between Adrb2(-/-) and WT DMM as well as Adrb2(-/-) and WT Sham mice. Number of alpha 2-AR-positive cells was lower in Adrb2(-/-) than in WT mice in all analyzed tissues and decreased in both Adrb2(-/-) and WT over time. Conclusion: We propose that the increased bone mass in Adrb2(-/-) DMM mice was not only due to beta 2-AR deficiency but to a synergistic effect of OA and elevated leptin concentrations. Taken together, beta 2-AR plays a major role in OA-related subchondral bone remodeling and is thus an attractive target for the exploration of novel therapeutic avenues.