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Böhm, Daniel ; Koall, Martin ; Matysik, Frank‐Michael

Dead volume–free flow splitting in capillary electrophoresis

Böhm, Daniel, Koall, Martin and Matysik, Frank‐Michael (2022) Dead volume–free flow splitting in capillary electrophoresis. Electrophoresis 43 (13-14), pp. 1438-1445.

Date of publication of this fulltext: 26 Jul 2022 09:16
Article
DOI to cite this document: 10.5283/epub.52635


Abstract

In recent years, several dual detection concepts (DDCs) for CE were developed, which consisted of at least one nondestructive detector. For these DDCs, a linear detector arrangement could be used, which is not possible when both detectors are destructive. To overcome this problem, we developed a concept for the splitting of the CE stream utilizing commercially available flow splitters (FSs) that ...

In recent years, several dual detection concepts (DDCs) for CE were developed, which consisted of at least one nondestructive detector. For these DDCs, a linear detector arrangement could be used, which is not possible when both detectors are destructive. To overcome this problem, we developed a concept for the splitting of the CE stream utilizing commercially available flow splitters (FSs) that allow the parallel positioning of two destructive detectors. In this proof-of-concept study, T- and Y-shaped FSs were characterized regarding their suitability for DDCs. To keep it simple, a UV detector (UV) and a (CD)-D-4 were used for the characterization. The model system consisted of an acetonitrile-based background electrolyte and the two model substances, (ferrocenylmethyl)trimethylammonium iodide and caffeine. CE hyphenated to a UV detector (CE-UV) measurements revealed that the split ratio was about 50% for both FSs. CE-(CD)-D-4 was used to evaluate the peak shape in front of and behind the FSs. These measurements showed that there was no significant peak broadening introduced by the FSs. Additionally, there were no changes in the LODs in front of and behind the FSs. Furthermore, the flexibility of the new FS approach allowed the usage of capillaries with different ids (25-75 mu m) for injection and detection.



Involved Institutions


Details

Item typeArticle
Journal or Publication TitleElectrophoresis
Publisher:Wiley
Open Access Type:DEAL (Wiley)
Place of Publication:HOBOKEN
Volume:43
Number of Issue or Book Chapter:13-14
Page Range:pp. 1438-1445
Date25 March 2022
InstitutionsChemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik)
Projects
Funded by: Deutsche Forschungsgemeinschaft (DFG) (441791263)
Identification Number
ValueType
10.1002/elps.202200025DOI
KeywordsCONTACTLESS CONDUCTIVITY DETECTION; MASS-SPECTROMETRIC DETECTION; ELECTROCHEMICAL DETECTION; SYSTEM; TRENDS; capillary electrophoresis; dead volume-free; dual detection; flow splitting; various capillaries inside diameter
Dewey Decimal Classification500 Science > 540 Chemistry & allied sciences
StatusPublished
RefereedYes, this version has been refereed
Created at the University of RegensburgYes
URN of the UB Regensburgurn:nbn:de:bvb:355-epub-526352
Item ID52635

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