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Böhm, Daniel ; Koall, Martin ; Matysik, Frank‐Michael

Dead volume–free flow splitting in capillary electrophoresis

Böhm, Daniel, Koall, Martin und Matysik, Frank‐Michael (2022) Dead volume–free flow splitting in capillary electrophoresis. Electrophoresis 43 (13-14), S. 1438-1445.

Veröffentlichungsdatum dieses Volltextes: 26 Jul 2022 09:16
Artikel
DOI zum Zitieren dieses Dokuments: 10.5283/epub.52635


Zusammenfassung

In recent years, several dual detection concepts (DDCs) for CE were developed, which consisted of at least one nondestructive detector. For these DDCs, a linear detector arrangement could be used, which is not possible when both detectors are destructive. To overcome this problem, we developed a concept for the splitting of the CE stream utilizing commercially available flow splitters (FSs) that ...

In recent years, several dual detection concepts (DDCs) for CE were developed, which consisted of at least one nondestructive detector. For these DDCs, a linear detector arrangement could be used, which is not possible when both detectors are destructive. To overcome this problem, we developed a concept for the splitting of the CE stream utilizing commercially available flow splitters (FSs) that allow the parallel positioning of two destructive detectors. In this proof-of-concept study, T- and Y-shaped FSs were characterized regarding their suitability for DDCs. To keep it simple, a UV detector (UV) and a (CD)-D-4 were used for the characterization. The model system consisted of an acetonitrile-based background electrolyte and the two model substances, (ferrocenylmethyl)trimethylammonium iodide and caffeine. CE hyphenated to a UV detector (CE-UV) measurements revealed that the split ratio was about 50% for both FSs. CE-(CD)-D-4 was used to evaluate the peak shape in front of and behind the FSs. These measurements showed that there was no significant peak broadening introduced by the FSs. Additionally, there were no changes in the LODs in front of and behind the FSs. Furthermore, the flexibility of the new FS approach allowed the usage of capillaries with different ids (25-75 mu m) for injection and detection.



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Details

DokumentenartArtikel
Titel eines Journals oder einer ZeitschriftElectrophoresis
Verlag:Wiley
Ort der Veröffentlichung:HOBOKEN
Band:43
Nummer des Zeitschriftenheftes oder des Kapitels:13-14
Seitenbereich:S. 1438-1445
Datum25 März 2022
InstitutionenChemie und Pharmazie > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik)
Projekte
Gefördert von: Deutsche Forschungsgemeinschaft (DFG) (441791263)
Identifikationsnummer
WertTyp
10.1002/elps.202200025DOI
Stichwörter / KeywordsCONTACTLESS CONDUCTIVITY DETECTION; MASS-SPECTROMETRIC DETECTION; ELECTROCHEMICAL DETECTION; SYSTEM; TRENDS; capillary electrophoresis; dead volume-free; dual detection; flow splitting; various capillaries inside diameter
Dewey-Dezimal-Klassifikation500 Naturwissenschaften und Mathematik > 540 Chemie
StatusVeröffentlicht
BegutachtetJa, diese Version wurde begutachtet
An der Universität Regensburg entstandenJa
URN der UB Regensburgurn:nbn:de:bvb:355-epub-526352
Dokumenten-ID52635

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