Is the In Vitro Observed NETosis the Favored Physiological Death of Neutrophils or Mainly Induced by an Isolation Bias?

URN to cite this document:: urn:nbn:de:bvb:355-epub-540966
DOI to cite this document:: 10.5283/epub.54096

Rimboeck, Julia

; Gruber, Michael

; Wittmann, Sigrid

License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 20 Apr 2023 14:28

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Abstract

Centrifugation steps are regularly used for neutrophil isolation. Thereby, the influences of applied g-forces on the functionality of PMNs have hardly been analyzed and could consequently have been overlooked or led to biased results. We now hypothesize that blood PMNs-when gently isolated-can be long-lived cells and they physiologically become apoptotic rather than NETotic. Neutrophils were ...