A rapid protocol for ribosome profiling of low input samples

URN to cite this document:: urn:nbn:de:bvb:355-epub-546123
DOI to cite this document:: 10.5283/epub.54612

Meindl, Andreas ; Romberger, Markus ; Lehmann, Gerhard ; Eichner, Norbert ; Kleemann, Leon ; Wu, Jie ; Danner, Johannes ; Boesl, Maria ; Mesitov, Mikhail ; Meister, Gunter

; König, Julian ; Leidel, Sebastian Andreas ; Medenbach, Jan

License: Creative Commons Attribution Non-commercial 4.0
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Date of publication of this fulltext: 04 Sep 2023 13:59

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Abstract

Ribosome profiling provides quantitative, comprehensive, and high-resolution snapshots of cellular translation by the high-throughput sequencing of short mRNA fragments that are protected by ribosomes from nucleolytic digestion. While the overall principle is simple, the workflow of ribosome profiling experiments is complex and challenging, and typically requires large amounts of sample, limiting ...