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A rapid protocol for ribosome profiling of low input samples

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Meindl, Andreas ; Romberger, Markus ; Lehmann, Gerhard ; Eichner, Norbert ; Kleemann, Leon ; Wu, Jie ; Danner, Johannes ; Boesl, Maria ; Mesitov, Mikhail ; Meister, Gunter ; König, Julian ; Leidel, Sebastian Andreas ; Medenbach, Jan
Date of publication of this fulltext: 04 Sep 2023 13:59


Ribosome profiling provides quantitative, comprehensive, and high-resolution snapshots of cellular translation by the high-throughput sequencing of short mRNA fragments that are protected by ribosomes from nucleolytic digestion. While the overall principle is simple, the workflow of ribosome profiling experiments is complex and challenging, and typically requires large amounts of sample, limiting ...


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