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Phot-LOV1: Photocycle of a blue-light receptor domain from the green alga Chlamydomonas reinhardtii

Kottke, Tilman ; Heberle, Joachim ; Hehn, Dominic ; Dick, Bernhard ; Hegemann, Peter


The \"Phot\" protein family comprises blue-light photoreceptors that consist of two FMN-binding LOV (light, oxygen, and voltage) domains and a serine/threonine kinase domain. We have investigated the LOV1 domain of Phot1 from Chlamydomonas reinhardtii by time-resolved absorption spectroscopy. Photoexcitation of the dark form, LOV1-447, causes transient bleaching and formation of two spectrally ...


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