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Versatile workflow for cell type–resolved transcriptional and epigenetic profiles from cryopreserved human lung

Llamazares-Prada, Maria ; Espinet, Elisa ; Mijošek, Vedrana ; Schwartz, Uwe ; Lutsik, Pavlo ; Tamas, Raluca ; Richter, Mandy ; Behrendt, Annika ; Pohl, Stephanie T. ; Benz, Naja P. ; Muley, Thomas ; Warth, Arne ; Heußel, Claus Peter ; Winter, Hauke ; Landry, Jonathan J. M. ; Herth, Felix J.F. ; Mertens, Tinne C.J. ; Karmouty-Quintana, Harry ; Koch, Ina ; Benes, Vladimir ; Korbel, Jan O. ; Waszak, Sebastian M. ; Trumpp, Andreas ; Wyatt, David M. ; Stahl, Heiko F. ; Plass, Christoph ; Jurkowska, Renata Z.


Complexity of lung microenvironment and changes in cellular composition during disease make it exceptionally hard to understand molecular mechanisms driving development of chronic lung diseases. Although recent advances in cell type-resolved approaches hold great promise for studying complex diseases, their implementation relies on local access to fresh tissue, as traditional tissue storage ...


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