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Recombinant Histidine-Tagged Nano-protein-based Highly Sensitive Electro-Sensing Device for Salivary Cortisol

Ganpat Chavan, Sachin ; Kumar Yagati, Ajay ; Koyappayil, Aneesh ; Go, Anna ; Yeon, Sangho ; Lee, Min-Ho


We have developed a powerful biosensing strategy for immobilizing histidine-tagged (His-Tag)-oriented recombinant nano-protein immobilization on a chemically modified glassy carbon electrode (GCE) surfaces via (S)-N-(5-amino-1-carboxypentyl)iminodiacetic acid (ANTA) acting as a chelating Ni2' centered interaction. Here, we introduce a label-free electro-sensor to quantify cortisol levels in ...


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