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- URN zum Zitieren dieses Dokuments:
- urn:nbn:de:bvb:355-epub-578684
- DOI zum Zitieren dieses Dokuments:
- 10.5283/epub.57868
Zusammenfassung
Nucleoside analogues like 4-thiouridine (4sU) are used to metabolically label newly synthesized RNA. Chemical conversion of 4sU before sequencing induces T-to-C mismatches in reads sequenced from labelled RNA, allowing to obtain total and labelled RNA expression profiles from a single sequencing library. Cytotoxicity due to extended periods of labelling or high 4sU concentrations has been ...
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