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Correcting 4sU induced quantification bias in nucleotide conversion RNA-seq data

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Berg, Kevin ; Lodha, Manivel ; Delazer, Isabel ; Bartosik, Karolina ; Garcia, Yilliam Cruz ; Hennig, Thomas ; Wolf, Elmar ; Dölken, Lars ; Lusser, Alexandra ; Prusty, Bhupesh K. ; Erhard, Florian
Date of publication of this fulltext: 07 Mar 2024 13:43


Nucleoside analogues like 4-thiouridine (4sU) are used to metabolically label newly synthesized RNA. Chemical conversion of 4sU before sequencing induces T-to-C mismatches in reads sequenced from labelled RNA, allowing to obtain total and labelled RNA expression profiles from a single sequencing library. Cytotoxicity due to extended periods of labelling or high 4sU concentrations has been ...


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