HAHNE, JENS C. ; MEYER, SUSANNE R. ; GAMBARYAN, STEPAN 
; WALTER, ULRICH ; DIETL, JOHANNES ; ENGEL, JÖRG B. ; HONIG, ARND
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
|---|
| Titel eines Journals oder einer Zeitschrift: | International Journal of Oncology |
|---|
| Verlag: | SPANDIDOS PUBL LTD |
|---|
| Ort der Veröffentlichung: | ATHENS |
|---|
| Band: | 42 |
|---|
| Nummer des Zeitschriftenheftes oder des Kapitels: | 5 |
|---|
| Seitenbereich: | S. 1630-1635 |
|---|
| Datum: | 2013 |
|---|
| Institutionen: | Medizin > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
|---|
| Identifikationsnummer: | | Wert | Typ |
|---|
| 10.3892/ijo.2013.1846 | DOI |
|
|---|
| Stichwörter / Keywords: | PLATINUM RESISTANCE; PATHWAYS; IMMUNOSURVEILLANCE; INHIBITION; EXPRESSION; ROLES; LINES; GENE; ovarian cancer; AKT; platinum-resistance; natural-killer cells |
|---|
| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
|---|
| Status: | Veröffentlicht |
|---|
| Begutachtet: | Ja, diese Version wurde begutachtet |
|---|
| An der Universität Regensburg entstanden: | Ja |
|---|
| Dokumenten-ID: | 62613 |
|---|
Web of Science
Zusammenfassung
Platinum-resistance is the most crucial problem for treatment of ovarian cancer. There is a clinical need for new treatment strategies which overcome platinum resistance. As survival is strongly influenced by immunological parameters, immunotherapeutic strategies appear promising. Therefore a better understanding of the interaction between ovarian tumour cells and cells of the immune system is a ...
Zusammenfassung
Platinum-resistance is the most crucial problem for treatment of ovarian cancer. There is a clinical need for new treatment strategies which overcome platinum resistance. As survival is strongly influenced by immunological parameters, immunotherapeutic strategies appear promising. Therefore a better understanding of the interaction between ovarian tumour cells and cells of the immune system is a necessary prerequisite. In the present study we aimed to enlighten the interactions between platinum resistant and platinum sensitive ovarian cancer cells and natural-killer (NK)-cells. Modified FATAL assay was used for determining the killing efficiency of NK-cells for the parental A2780 cells and the cis-platinum resistant A2780cis human ovarian cancer cells. Expression of pro- and anti-apoptotic genes as well as ligands involved in NK-cell receptor recognition were analysed by RT-PCR and flow cytometric analysis. The efficiency of NK mediated cell lysis differs between A2780 cells and the cis-platinum-resistant A2780cis cells. A2780cis cells are less accessible for NK-cell mediated killing. Based on this observation we characterized the molecular basis for resistance mechanisms. Besides an increase in anti-apoptotic genes (especially CIAP-1 and -2) that probably render A2780cis cells more resistant against apoptosis an increased amount of soluble MICA/B seems to be responsible for the lower killing rate of platinum-resistant A2780cis cells compared to their parental A2780 cells.
Altmetric