Zusammenfassung
We report on the colorimetric and fluorimetric responses of the cationic water-soluble polythiophene derivative poly[3-(2-(N-(N'-methylimidazole))ethoxy)-4-methylthiophene] in presence of different phosphates with a focus on ATP. UV/Vis absorbance and fluorescence measurements show that ATP concentrations can be determined in aqueous solutions with a sufficient selectivity to other phosphates. ...
Zusammenfassung
We report on the colorimetric and fluorimetric responses of the cationic water-soluble polythiophene derivative poly[3-(2-(N-(N'-methylimidazole))ethoxy)-4-methylthiophene] in presence of different phosphates with a focus on ATP. UV/Vis absorbance and fluorescence measurements show that ATP concentrations can be determined in aqueous solutions with a sufficient selectivity to other phosphates. Measurements of circular dichroism reveal that the polymer forms a random coil structure that exhibits no signal in the CD spectrum. Upon addition of ATP the conformation changes to a helix, yielding in strong CD peaks. These experimental data suggest that this polymer is an attractive, simple and versatile colorimetric and fluorimetric probe for nucleoside triphosphates such as ATP. (C) 2012 Elsevier Ltd. All rights reserved.
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