| Dokumentenart: | Artikel | ||||
|---|---|---|---|---|---|
| Titel eines Journals oder einer Zeitschrift: | Journal of Fluorescence | ||||
| Verlag: | SPRINGER/PLENUM PUBLISHERS | ||||
| Ort der Veröffentlichung: | NEW YORK | ||||
| Band: | 22 | ||||
| Nummer des Zeitschriftenheftes oder des Kapitels: | 2 | ||||
| Seitenbereich: | S. 659-676 | ||||
| Datum: | 2012 | ||||
| Institutionen: | Chemie und Pharmazie > Institut für Analytische Chemie, Chemo- und Biosensorik | ||||
| Identifikationsnummer: |
| ||||
| Stichwörter / Keywords: | SQUARE-WAVE VOLTAMMETRY; ORGANOPHOSPHORUS PESTICIDES; ENERGY-TRANSFER; EUROPIUM; EMISSION; SYSTEMS; ASSAYS; Europium-3-carboxycoumarin; Time resolved fluorescence; Pesticides aldicarb; Methomyl; Prometryne; Cyclic voltammetry; Differential pulse voltammetry; Linear sweep voltammetry; Glassy carbon electrode | ||||
| Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 540 Chemie | ||||
| Status: | Veröffentlicht | ||||
| Begutachtet: | Ja, diese Version wurde begutachtet | ||||
| An der Universität Regensburg entstanden: | Ja | ||||
| Dokumenten-ID: | 63964 |
Web of ScienceZusammenfassung
This work describes the application of time resolved fluorescence in microtiterplates and electrochemical methods on glassy carbon electrode for investigating the interactions of europium-3-carboxycoumarin with pesticides aldicarb, methomyl and prometryne. Stern-volmer studies at different temperatures indicate that static quenching dominates for methomyl, aldicarb and prometryne. By using ...
Zusammenfassung
This work describes the application of time resolved fluorescence in microtiterplates and electrochemical methods on glassy carbon electrode for investigating the interactions of europium-3-carboxycoumarin with pesticides aldicarb, methomyl and prometryne. Stern-volmer studies at different temperatures indicate that static quenching dominates for methomyl, aldicarb and prometryne. By using Lineweaver-Burk equation binding constants were determined at 303 K, 308 K and 313 K. A thermodynamic analysis showed that the reaction is spontaneous with Delta G being negative. The enthalpy Delta H and the entropy Delta S of reactions were all determined. A time-resolved (gated) luminescence-based method for determination of pesticides in microtiterplate format using the long-lived europium-3-carboxycoumarin has been developed. The limit of detection is 4.80, 5.06 and 8.01 mu mol L-1 for methomyl, prometryne and aldicarb, respectively. This is the lowest limit of detection achieved so far for luminescent lanthanide-based probes for pesticides. The interaction of the probe with the pesticides has been investigated using cyclic voltammetry (CV), differential pulse polarography (DPP), square wave voltammetry (SWV) and linear sweep voltammetry (LSV) on a glassy carbon electrode in I = 0.1 mol L-1 p-toluenesulfonate at 25 A degrees C. The diffusion coefficients of the reduced species are calculated. The main properties of the electrode reaction occurring in a finite diffusion space are the quasireversible maximum and the splitting of the net SWV peak for Eu(III) ions in the ternary complex formed . It was observed that the increase of the cathodic peak currents using LSV is linear with the increase of pesticides concentration in the range 5 x 10(-7) to 1 x 10(-5) mol L-1. The detection limit (DL) were about 1.01, 2.23 and 1.89 mu molL(-1) for aldicarb, methomyl and prometryne, respectively. In order to assess the analytical applicability of the method, the influence of various potentially interfering species was examined. Influence of interfering species on the recovery of 10 mu mol L-1 pesticides has been investigated.
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