Zusammenfassung
Methods: Determination of 4-ABP and o-toluidine-releasing DNA adducts in epithelial and submucosal bladder tissues of sudden death victims (SDV: n == 46), and bladder tumours (n == 12) by gas chromatography/mass spectrometry. Results: Above background, 4 and 11 of 12 tumour samples contained adducts of 4-ABP (0.057 +/-+/- 0.125 fmol/mu A mu g DNA) and o-toluidine (8.72 +/-+/- 4.49 fmol/mu A mu g ...
Zusammenfassung
Methods: Determination of 4-ABP and o-toluidine-releasing DNA adducts in epithelial and submucosal bladder tissues of sudden death victims (SDV: n == 46), and bladder tumours (n == 12) by gas chromatography/mass spectrometry. Results: Above background, 4 and 11 of 12 tumour samples contained adducts of 4-ABP (0.057 +/-+/- 0.125 fmol/mu A mu g DNA) and o-toluidine (8.72 +/-+/- 4.49 fmol/mu A mu g DNA), respectively. Lower adduct levels were present in both epithelial and submucosal bladder tissues of SDV (4-ABP: 0.011 +/-+/- 0.022 and 0.019 +/-+/- 0.047 fmol/mu A mu g DNA; o-toluidine: 0.24 +/-+/- 0.63 and 0.27 +/-+/- 0.70 fmol/mu A mu g DNA). Conclusion: Detection of o-toluidine-releasing DNA adducts support the carcinogenicity of o-toluidine in the human bladder.
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