Zusammenfassung
Periosteal tissue is a source of growth factors and of osteochondral progenitor cells which makes it suitable for implantation in chondral defects as known in autologous chondrocyte implantation The aim of this study was to determine the interaction between periosteal tissue and articular chondrocytes with respect to catabolic effectors such as matrix metalloproteinases (MMPs) and IL 6 Human ...
Zusammenfassung
Periosteal tissue is a source of growth factors and of osteochondral progenitor cells which makes it suitable for implantation in chondral defects as known in autologous chondrocyte implantation The aim of this study was to determine the interaction between periosteal tissue and articular chondrocytes with respect to catabolic effectors such as matrix metalloproteinases (MMPs) and IL 6 Human articular chondrocytes were cultured for up to 28 days as micromass pellets in coculture either with physical contact to periosteal explants or allowing paracrine interactions only Expression, secretion and activation of MMPs and IL 6 were analyzed m chondrocytes, periosteum, and culture supernatants Both coculture conditions influence gene expression levels of MMPs and IL 6 in a time culture, and tissue dependent manner Coculturing of periosteum with chondrocytes promotes gene expression and secretion of IL 6 In periosteum physical contact inhibits MMP 2 and MMP 13 gene expression while paracrine coculture induces expression of IL 6 MMP 2, 7, and 13 Pro MMP 2, 7 and 13 were detected in supernatants of all culture regimens whereas pro MMP 9 was secreted from periosteum only As a balanced amount of MMP activity is likely required to achieve sufficient integration of the regenerate tissue with the surrounding healthy cartilage, an exceeding expression of proteinases might result in degradation, hypertrophy or rejection of the graft (c) 2010 Orthopaedic Research Society Published by Wiley Periodicals, Inc J Orthop Res 28 1576-1585 2010
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