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Microtiterplate phosphate assay based on luminescence quenching of a terbium complex amenable to decay time detection

Turel, Matejka ; Duerkop, Axel ; Yegorova, Alla ; Karasyov, Alexander ; Scripinets, Yulia ; Lobnik, Aleksandra



Zusammenfassung

We describe a terbium-ligand complex (TbL) for a microtiterplate assay for phosphate (P) in the 0.3-100 mu mol L-1 range based on luminescence quenching. As the pH optimum is at neutral pH (7.4) the probe is quenched by both, primary (H2PO4-) and secondary phosphate (HPO42-). The LOD is 110 nmol L-1. A Stern-Volmer study revealed that quenching is mostly static. Due to the ms-decay time of TbL, ...

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