Kubosaki, Atsutaka ; Lindgren, Gabriella ; Tagami, Michihira ; Simon, Christophe ; Tomaru, Yasuhiro ; Miura, Hisashi ; Suzuki, Takahiro 
; Arner, Erik ; Forrest, Alistair R.R. ; Irvine, Katharine M. ; Schroder, Kate ; Hasegawa, Yuki ; Kanamori-Katayama, Mutsumi ; Rehli, Michael ; Hume, David A. ; Kawai, Jun ; Suzuki, Masanori ; Suzuki, Harukazu ; Hayashizaki, Yoshihide
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Molecular Immunology |
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| Verlag: | PERGAMON-ELSEVIER SCIENCE LTD |
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| Ort der Veröffentlichung: | OXFORD |
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| Band: | 47 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 14 |
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| Seitenbereich: | S. 2295-2302 |
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| Datum: | 2010 |
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| Institutionen: | Medizin > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1016/j.molimm.2010.05.289 | DOI |
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| Stichwörter / Keywords: | SEQUENCE-BINDING-PROTEIN; REGULATORY FACTOR FAMILY; TRANSCRIPTION FACTORS; MYELOID CELLS; INTERFERON; PU.1; MACROPHAGES; DIFFERENTIATION; EXPRESSION; MOUSE; ChIp-chip; IRF8; PU 1; Interferon |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 65915 |
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Web of Science
Zusammenfassung
Gene regulatory networks in living cells are controlled by the interaction of multiple cell type-specific transcription regulators with DNA binding sites in target genes Interferon regulatory factor 8 (IRF8) also known as Interferon consensus sequence binding protein (ICSBP) is a transcription factor expressed predominantly in myeloid and lymphoid cell lineages To find the functional direct ...
Zusammenfassung
Gene regulatory networks in living cells are controlled by the interaction of multiple cell type-specific transcription regulators with DNA binding sites in target genes Interferon regulatory factor 8 (IRF8) also known as Interferon consensus sequence binding protein (ICSBP) is a transcription factor expressed predominantly in myeloid and lymphoid cell lineages To find the functional direct target genes of IRF8 the gene expression profiles of siRNA knockdown samples and genome-wide binding locations by ChIP-chip were analyzed in THP-1 myelomonocytic leukemia cells Consequently 84 genes were identified as functional direct targets The ETS family transcription factor PU 1 also known as SPI1 binds to IRF8 and regulates basal transcription in macrophages Using the same approach we identified 53 direct target genes of PU 1 these overlapped with 19 IRF8 targets These 19 genes included key molecules of IFN signaling such as OAS1 and IRF9 but excluded other IFN-related genes amongst the IRF8 functional direct target genes We suggest that IRF8 and PU 1 can have both combined and independent actions on different promoters in myeloid cells (C) 2010 Elsevier Ltd All rights reserved
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