Dango, Sebastian ; Wang, Xiao Tao ; Gold, Monika ; Cucuruz, Beatrix ; Klein, Christoph A. ; Passlick, Bernward ; Sienel, Wulf
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Lung Cancer |
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| Verlag: | ELSEVIER IRELAND LTD |
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| Ort der Veröffentlichung: | CLARE |
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| Band: | 67 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 3 |
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| Seitenbereich: | S. 290-295 |
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| Datum: | 2010 |
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| Institutionen: | Medizin > Lehrstuhl für Pathologie |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1016/j.lungcan.2009.04.012 | DOI |
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| Stichwörter / Keywords: | POLYMERASE CHAIN-REACTION; VINORELBINE PLUS CISPLATIN; METASTATIC MELANOMA; GENE; IDENTIFICATION; CARCINOMA; PROLIFERATION; CHEMOTHERAPY; PROGRESSION; DIAGNOSIS; Non-small cell lung cancer (NSCLC); Lymph node metastasis; Melanoma-antigen (MAGE); Disseminated tumor cells (DTCs); Quantitative RT-PCR; Adjuvant immunotherapy |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 66349 |
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Web of Science
Zusammenfassung
Purpose. Single disseminated tumor cells are detectable in regional lymph nodes of 30-50% patients with early-stage non-small cell lung cancer (NSCLC) This study investigated if these disseminated tumor cells express MACE-A and thus might be targeted by adjuvant anti-MACE-A immunotherapies. Experimental design Lymph nodes of 32 consecutive patients without neoadjuvant therapy were removed by ...
Zusammenfassung
Purpose. Single disseminated tumor cells are detectable in regional lymph nodes of 30-50% patients with early-stage non-small cell lung cancer (NSCLC) This study investigated if these disseminated tumor cells express MACE-A and thus might be targeted by adjuvant anti-MACE-A immunotherapies. Experimental design Lymph nodes of 32 consecutive patients without neoadjuvant therapy were removed by systematic lymphadenectomy during resection of NSCLC One-hundred of these lymph nodes were cut Into two equal halves which were examined using either routine histo-pathology or quantitative reverse transcriptase PCR (qRT-PCR) qRT-PCR amplification of cytokeratin 19 transcripts was applied for the detection of disseminated tumor cells Expression of MACE-A was analyzed using one single primer pair amplifying subgroups MACE-A1 to -A6 in one qRT-PCR reaction Results. Ninety-four (94%) lymph nodes were tumor-free by histo-pathology qRT-PCR detected disseminated tumor cells in 26 (28%) of these lymph nodes resulting in 19 (59%) patients with disseminated tumor cells. All of the remaining 6 lymph nodes that were judged by the pathologist to contain tumor cells exhibited CK19 transcripts Fifteen (46%) lymph nodes with disseminated tumor cells contained MACE-A transcripts resulting in 12 (37%) patients with disseminated tumor cells which expressed MAGE-A There was no correlation between clinico-pathological parameters and the occurrence of disseminated tumor cells or their MACE-A expression Conclusions. Since 37% of patients with operable NSCLC harbored disseminated tumor cells that expressed MACE-A, only these patients might benefit from adjuvant immunotherapies directed against MAGE-A1 to -A6. This study may provide a basis for the preselection of patients to be Included in such immunotherapy trials after resection of NSCLC (C) 2009 Elsevier Ireland Ltd All rights reserved
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