Kopp, Andrea ; Bala, Margarita ; Weigert, Johanna ; Büchler, Christa ; Neumeier, Markus ; Aslanidis, Charalampos ; Schölmerich, Jürgen ; Schäffler, Andreas
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Cytokine |
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| Verlag: | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
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| Ort der Veröffentlichung: | LONDON |
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| Band: | 49 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 1 |
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| Seitenbereich: | S. 51-57 |
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| Datum: | 2010 |
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| Institutionen: | Medizin > Lehrstuhl für Innere Medizin I
Medizin > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1016/j.cyto.2009.10.001 | DOI |
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| Stichwörter / Keywords: | TUMOR-NECROSIS-FACTOR; REPEAT-CONTAINING SEQUENCE; HIGH-DENSITY-LIPOPROTEIN; COLLAGENOUS REPEAT; 26-KDA PROTEIN; FACTOR-ALPHA; CHROMOSOMAL LOCALIZATION; PROMOTES PROLIFERATION; GENOMIC ORGANIZATION; ADIPOSE-TISSUE; Monocyte; CTRP-3; Type 2 diabetes mellitus |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 66535 |
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Web of Science
Zusammenfassung
Aims/Hypothesis: It was the aim to investigate the hypothesis that the new C1q/TNF-family member CTRP-3 (C1q/TNF-related protein-3) acts anti-inflammatory in human monocytes from healthy controls and patients with type 2 diabetes mellitus (T2D). Methods: Monocytes were isolated from 20 healthy controls and 30 patients with T2D. IL-6 and TNF concentrations were measured by ELISA. CTRP-3 was ...
Zusammenfassung
Aims/Hypothesis: It was the aim to investigate the hypothesis that the new C1q/TNF-family member CTRP-3 (C1q/TNF-related protein-3) acts anti-inflammatory in human monocytes from healthy controls and patients with type 2 diabetes mellitus (T2D). Methods: Monocytes were isolated from 20 healthy controls and 30 patients with T2D. IL-6 and TNF concentrations were measured by ELISA. CTRP-3 was expressed in insect cells and used for stimulation experiments. Results: Basal IL-6 and TNF were not different in control and in T2D monocytes. LPS-stimulation (1 mu g/ml) significantly (p < 0.001) increased IL-6 and TNF in the supernatants of control and in T2D monocytes to a similar extent. CTRP-3 (1 mu g/ml) significantly (p = 0.03) inhibited LPS-induced IL-6 in control monocytes but not in T2D monocytes. TNF upon co-stimulation with LPS and CTRP-3 was significantly (p = 0.012) lower in control than in T2D monocytes. LPS-induced TNF concentration was significantly and positively correlated with serum total cholesterol and LDL cholesterol in T2D patients. Conclusions: CTRP-3 inhibits LPS-induced IL-6 and TNF release. This anti-inflammatory effect is lost in T2D. Serum cholesterol concentration affects the pro-inflammatory potential of LPS to induce TNF release from T2D monocytes in the presence or absence of CTRP-3. CTRP-3 might partly account for the pro-inflammatory state in T2D. (C) 2009 Elsevier Ltd. All rights reserved.
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