Dokumentenart: | Artikel | ||||
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Titel eines Journals oder einer Zeitschrift: | PLoS Medicine | ||||
Verlag: | PUBLIC LIBRARY SCIENCE | ||||
Ort der Veröffentlichung: | SAN FRANCISCO | ||||
Band: | 6 | ||||
Nummer des Zeitschriftenheftes oder des Kapitels: | 7 | ||||
Seitenbereich: | e1000113 | ||||
Datum: | 2009 | ||||
Institutionen: | Medizin > Lehrstuhl für Innere Medizin I | ||||
Identifikationsnummer: |
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Stichwörter / Keywords: | CENTRAL-NERVOUS-SYSTEM; MYELOID SUPPRESSOR-CELLS; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; ANTIGEN-PRESENTING CELLS; MYELIN BASIC-PROTEIN; CD8(+) T-CELLS; INFLAMMATORY MONOCYTES; ALZHEIMERS-DISEASE; PROMOTES RECOVERY; DENDRITIC CELLS; | ||||
Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin | ||||
Status: | Veröffentlicht | ||||
Begutachtet: | Ja, diese Version wurde begutachtet | ||||
An der Universität Regensburg entstanden: | Ja | ||||
Dokumenten-ID: | 67089 |
Zusammenfassung
Background: Although macrophages (M Phi) are known as essential players in wound healing, their contribution to recovery from spinal cord injury (SCI) is a subject of debate. The difficulties in distinguishing between different M Phi subpopulations at the lesion site have further contributed to the controversy and led to the common view of M Phi as functionally homogenous. Given the massive ...
Zusammenfassung
Background: Although macrophages (M Phi) are known as essential players in wound healing, their contribution to recovery from spinal cord injury (SCI) is a subject of debate. The difficulties in distinguishing between different M Phi subpopulations at the lesion site have further contributed to the controversy and led to the common view of M Phi as functionally homogenous. Given the massive accumulation in the injured spinal cord of activated resident microglia, which are the native immune occupants of the central nervous system (CNS), the recruitment of additional infiltrating monocytes from the peripheral blood seems puzzling. A key question that remains is whether the infiltrating monocyte-derived M Phi contribute to repair, or represent an unavoidable detrimental response. The hypothesis of the current study is that a specific population of infiltrating monocyte-derived M Phi is functionally distinct from the inflammatory resident microglia and is essential for recovery from SCI. Methods and Findings: We inflicted SCI in adult mice, and tested the effect of infiltrating monocyte-derived M Phi on the recovery process. Adoptive transfer experiments and bone marrow chimeras were used to functionally distinguish between the resident microglia and the infiltrating monocyte-derived M Phi. We followed the infiltration of the monocyte-derived M Phi to the injured site and characterized their spatial distribution and phenotype. Increasing the naive monocyte pool by either adoptive transfer or CNS-specific vaccination resulted in a higher number of spontaneously recruited cells and improved recovery. Selective ablation of infiltrating monocyte-derived M Phi following SCI while sparing the resident microglia, using either antibody-mediated depletion or conditional ablation by diphtheria toxin, impaired recovery. Reconstitution of the peripheral blood with monocytes resistant to ablation restored the lost motor functions. Importantly, the infiltrating monocyte-derived M Phi displayed a local anti-inflammatory beneficial role, which was critically dependent upon their expression of interleukin 10. Conclusions: The results of this study attribute a novel anti-inflammatory role to a unique subset of infiltrating monocyte-derived M Phi in SCI recovery, which cannot be provided by the activated resident microglia. According to our results, limited recovery following SCI can be attributed in part to the inadequate, untimely, spontaneous recruitment of monocytes. This process is amenable to boosting either by active vaccination with a myelin-derived altered peptide ligand, which indicates involvement of adaptive immunity in monocyte recruitment, or by augmenting the naive monocyte pool in the peripheral blood. Thus, our study sheds new light on the long-held debate regarding the contribution of M Phi to recovery from CNS injuries, and has potentially far-reaching therapeutic implications.
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