Sugden, Bill ; Barabas, Sascha ; Gary, Regina ; Bauer, Tanja ; Lindner, Juha ; Lindner, Petra 
; Weinberger, Birgit ; Jilg, Wolfgang ; Wolf, Hans ; Deml, Ludwig
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | PLoS Pathogens |
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| Verlag: | PUBLIC LIBRARY SCIENCE |
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| Ort der Veröffentlichung: | SAN FRANCISCO |
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| Band: | 4 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 11 |
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| Seitenbereich: | e1000198 |
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| Datum: | 2008 |
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| Institutionen: | Medizin > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1371/journal.ppat.1000198 | DOI |
|
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| Stichwörter / Keywords: | CLASS-I PRESENTATION; T-CELL RESPONSES; TAT-FUSION PROTEINS; DENDRITIC CELLS; EXOGENOUS ANTIGEN; ADAPTIVE IMMUNITY; NUCLEAR ANTIGEN; TRANSDUCTION; LYMPHOCYTES; MOLECULES; |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 67760 |
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Web of Science
Zusammenfassung
Soluble extracellular proteins usually do not enter the endogenous human leukocyte antigen (HLA) I-dependent presentation pathway of antigen-presenting cells, strictly impeding their applicability for the re-stimulation of protein-specific CD8(+) cytotoxic T lymphocytes (CTL). Here we present for the Epstein-Barr virus (EBV) BZLF1 a novel strategy that facilitates protein translocation into ...
Zusammenfassung
Soluble extracellular proteins usually do not enter the endogenous human leukocyte antigen (HLA) I-dependent presentation pathway of antigen-presenting cells, strictly impeding their applicability for the re-stimulation of protein-specific CD8(+) cytotoxic T lymphocytes (CTL). Here we present for the Epstein-Barr virus (EBV) BZLF1 a novel strategy that facilitates protein translocation into antigen-presenting cells by its solubilisation in high molar urea and subsequent pulsing of cells in presence of low molar urea. Stimulation of PBMC from HLA-matched EBV-seropositive individuals with urea-treated BZLF1 but not untreated BZLF1 induces an efficient reactivation of BZLF1-specific CTL. Urea-treated BZLF1 (uBZLF1) enters antigen-presenting cells in a temperature-dependent manner by clathrin-mediated endocytosis and is processed by the proteasome into peptides that are bound to nascent HLA I molecules. Dendritic cells and monocytes but also B cells can cross-present uBZLF1 in vitro. The strategy described here has potential for use in the development of improved technologies for the monitoring of protein-specific CTL.
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