Maisch, Tim ; Worlicek, Christine ; Babilas, Philipp ; Landthaler, Michael ; Szeimies, Rolf‐Markus
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Experimental Dermatology |
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| Verlag: | WILEY-BLACKWELL |
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| Ort der Veröffentlichung: | MALDEN |
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| Band: | 17 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 10 |
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| Seitenbereich: | S. 813-820 |
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| Datum: | 2008 |
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| Institutionen: | Medizin > Lehrstuhl für Dermatologie und Venerologie |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1111/j.1600-0625.2008.00705.x | DOI |
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| Stichwörter / Keywords: | TRANSDERMAL DRUG-DELIVERY; HUMAN STRATUM-CORNEUM; PHOTODYNAMIC THERAPY; IN-VITRO; PERCUTANEOUS-ABSORPTION; PENETRATION ENHANCERS; TOPICAL FORMULATIONS; AMINOLEVULINIC ACID; DIMETHYL-SULFOXIDE; MOUSE SKIN; distribution; epidermis; photodynamic; porcine skin; short chain alcohol |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 67797 |
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Web of Science
Zusammenfassung
Topical photodynamic therapy with 5-aminolevulinic acid (5-ALA) is a newly established treatment modality for epithelial skin cancer. Skin distribution of different 20% 5-ALA formulations was investigated by detection of protoporphyrin IX (PpIX) using an ex vivo porcine skin model. Fluorescence of PpIX was first detectable at 2 h after application of a 5-ALA/HCl-alcohol solution, followed by a ...
Zusammenfassung
Topical photodynamic therapy with 5-aminolevulinic acid (5-ALA) is a newly established treatment modality for epithelial skin cancer. Skin distribution of different 20% 5-ALA formulations was investigated by detection of protoporphyrin IX (PpIX) using an ex vivo porcine skin model. Fluorescence of PpIX was first detectable at 2 h after application of a 5-ALA/HCl-alcohol solution, followed by a 5-ALA gel +/- 40% DMSO > 5-ALA lipophilic ointment > 5-ALA hydrophilic ointment at 4 h after application. Intensity of PpIX was 10-fold higher after 5-ALA/HCl-alcohol application in contrast to 5-ALA hydrophilic ointment. Maxima of PpIX fluorescence were measured between 18 and 24 h after application. PpIX accumulation induced by 5-ALA within the lower parts of the epidermis was increased in order of 5-ALA hydrophilic ointment < 5-ALA lipophilic ointment < 5-ALA gel w/o DMSO < 5-ALA gel with DMSO < 5-ALA/HCl-alcohol. Illumination of skin incubated with different 5-ALA formulations led to a marked increase of apoptotic cells in the epidermis depending on the penetration depth of the 5-ALA formulations. A formulation containing short chain alcohols have been observed to increase the permeation and distribution of 5-ALA. PpIX fluorescence was detected earlier yielding to photodynamic effective amounts of PpIX in the epidermis as compared with all other 5-ALA formulations used. These data emphasizes the potential of investigating properties of new formulations using such a full thickness porcine skin model.
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