Indole as an Artificial DNA Base Incorporated via an Acyclic 2′-Deoxyriboside Substitute

Zusammenfassung

Indole was synthetically incorporated into DNA as an artificial base at specific sites of duplex DNA. An acyclic substitute for the 2'-deoxyribose was applied in order to obtain a chemically stable nucleoside analogue that can be synthesized by a fast and facile procedure. Studies by methods of optical spectroscopy revealed that the indole base surrogate is intercalated into the DNA base stack and behaves as a universal base analogue with only little influence of the counterbase.