Zusammenfassung
Expression of integrin alpha 10 is initiated at the beginning of chondrogenesis and continues throughout cartilage development in adult cartilage. In our study, we aim to identify regulatory sequences that control the cell-type specific expression of the human integrin alpha 10 gene. Therefore, promoter constructs harboring 1139 bp 5' of the transcriptional start site of the human integrin alpha ...
Zusammenfassung
Expression of integrin alpha 10 is initiated at the beginning of chondrogenesis and continues throughout cartilage development in adult cartilage. In our study, we aim to identify regulatory sequences that control the cell-type specific expression of the human integrin alpha 10 gene. Therefore, promoter constructs harboring 1139 bp 5' of the transcriptional start site of the human integrin alpha 10 gene were analyzed. Our experiments localized a promoter region that directs high levels of expression specifically in chondrocytes. A sequence analysis detected three consensus AP-2 binding sites within this functional domain. Functionality of these sites was tested and confirmed by cotransfection of AP-2 in a luciferase reporter assay. Interestingly, EMSA identified AP-2 epsilon as the major AP-2 protein binding to the AP-2 consensus sequences. Additionally, Ets-1 was shown to be a positive regulator of the integrin alpha 10 expression whereas Sox9 was irrelevant. Taken together, these results suggest that AP-2 epsilon and Ets-1 are involved in the regulation of integrin 000 transcription in chondrocytes. (c) 2006 Elsevier Inc. All rights reserved.
Altmetric