Zusammenfassung
The aim of this study was to investigate whether all sizes of wear particles are capable of provoking inflammatory responses and whether there are different responses among different particle sizes. The knees of 40 female Balb/c mice were injected with polystyrene particles of three different diameters, 0.5 mu m, 2.0 mu m, and 75 mu m, using a 0.1% vol/vol concentration. Seven days after particle ...
Zusammenfassung
The aim of this study was to investigate whether all sizes of wear particles are capable of provoking inflammatory responses and whether there are different responses among different particle sizes. The knees of 40 female Balb/c mice were injected with polystyrene particles of three different diameters, 0.5 mu m, 2.0 mu m, and 75 mu m, using a 0.1% vol/vol concentration. Seven days after particle injection, assessment of the synovial microcirculation using intravital microscopy, and histologic examination, were done. All the mice injected with polystyrene particles had enhanced leukocyte-endothelial cell interactions and histologic scores regardless of particle size when compared with control animals injected with sterile phosphate buffered saline. Polystyrene particles 0.5 mu m in size provoked stronger membrane thickening and increased leukocyte-endothelial cell interactions than 75-mu m particles. The fraction of rolling leukocytes was enhanced in the 2.0-mu m particle group when compared with the 75-mu m particle group. These results indicate that polystyrene particles of all sizes (0.5 mu m, 2.0 mu m, and 75 mu m) are capable of inducing an inflammatory response. Small particles (0.5 mu m, 2.0 mu m) seem to provoke a stronger inflammatory response than larger particles (75 mu m) in conditions with equal particle volume.
Altmetric