HAUSMANN, M ; OBERMEIER, F ; SCHREITER, K ; SPOTTL, T ; FALK, W ; SCHÖLMERICH, J ; HERFARTH, H ; SAFTIG, P ; ROGLER, G 
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Clinical and Experimental Immunology |
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| Verlag: | WILEY |
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| Ort der Veröffentlichung: | HOBOKEN |
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| Band: | 136 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 1 |
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| Seitenbereich: | S. 157-167 |
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| Datum: | 2004 |
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| Institutionen: | Medizin > Lehrstuhl für Innere Medizin I |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1111/j.1365-2249.2004.02420.x | DOI |
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| Stichwörter / Keywords: | ANTIGEN PRESENTATION; BREAST-CANCER; EXTRACELLULAR-MATRIX; COSTIMULATORY MOLECULES; INTESTINAL MACROPHAGES; BONE-RESORPTION; CELLS; EXPRESSION; PROTEASES; MUCOSA; cathepsin D; inflammatory bowel disease; macrophages |
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| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 71645 |
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Web of Science
Zusammenfassung
Down-regulation of receptors involved in the recognition or transmission of inflammatory signals and a reduced responsiveness support the concept that macrophages are 'desensitized' during their differentiation in the intestinal mucosa. During inflammatory bowel disease (IBD) intestinal macrophages (IMACs) change to a reactive or 'aggressive' type. After having established a method of isolation ...
Zusammenfassung
Down-regulation of receptors involved in the recognition or transmission of inflammatory signals and a reduced responsiveness support the concept that macrophages are 'desensitized' during their differentiation in the intestinal mucosa. During inflammatory bowel disease (IBD) intestinal macrophages (IMACs) change to a reactive or 'aggressive' type. After having established a method of isolation and purification of IMACs, message for cathepsin D was one of the mRNAs we found to be up-regulated in a subtractive hybridization of Crohn's disease (CD) macrophages versus IMACs from control mucosa. The expression of cathepsin D in intestinal mucosa was analysed by immunohistochemistry in biopsies from IBD and control patients and in a mouse model of dextran sulphate sodium (DSS)-induced acute and chronic colitis. IMACs were isolated and purified from normal and inflamed mucosa by immunomagnetic beads armed with a CD33 antibody. RT-PCR was performed for cathepsin D mRNA. Results were confirmed by Northern blot and flow cytometrical analysis. Immunohistochemistry revealed a significant increase in the cathepsin D protein expression in inflamed intestinal mucosa from IBD patients compared to non-inflamed mucosa. No cathepsin D polymerase chain reaction (PCR) product could be obtained with mRNA from CD33-positive IMACs from normal mucosa. Reverse transcription (RT)-PCR showed an induction of mRNA for cathepsin D in purified IMACs from IBD patients. Northern blot and flow cytometry analysis confirmed these results. Cathepsin D protein was also found in intestinal mucosa in acute and chronic DSS-colitis but was absent in normal mucosa. This study shows that expression of cathepsin D is induced in inflammation-associated IMACs. The presence of cathepsin D might contribute to the mucosal damage in IBD.
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