John, Gernot T ; Goelling, Detlef ; Klimant, Ingo ; Schneider, Holger ; Heinzle, Elmar
Alternative Links zum Volltext:DOIVerlag
| Dokumentenart: | Artikel |
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| Titel eines Journals oder einer Zeitschrift: | Journal of Dairy Research |
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| Verlag: | CAMBRIDGE UNIV PRESS |
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| Ort der Veröffentlichung: | NEW YORK |
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| Band: | 70 |
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| Nummer des Zeitschriftenheftes oder des Kapitels: | 3 |
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| Seitenbereich: | S. 327-333 |
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| Datum: | 2003 |
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| Institutionen: | Chemie und Pharmazie > Institut für Analytische Chemie, Chemo- und Biosensorik |
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| Identifikationsnummer: | | Wert | Typ |
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| 10.1017/S0022029903006344 | DOI |
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| Stichwörter / Keywords: | STORAGE; YOGURT; optical pH sensing; microtitre plate; dairy starter culture; acid production; Lactococcus lactis; Streptococcus thermophilus |
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| Dewey-Dezimal-Klassifikation: | 500 Naturwissenschaften und Mathematik > 540 Chemie |
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| Status: | Veröffentlicht |
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| Begutachtet: | Ja, diese Version wurde begutachtet |
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| An der Universität Regensburg entstanden: | Ja |
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| Dokumenten-ID: | 72145 |
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Web of Science
Zusammenfassung
A new method for characterization of acid production by dairy starter cultures is presented. Microplates with integrated optical pH sensors are developed. Two fluorophores, a pH-sensitive and a pH-insensitive one are immobilised at the bottom of a polystyrene 96-well microtitre plate. The pH-insensitive fluorophore serves as an internal reference and makes calibration unnecessary. The sensor ...
Zusammenfassung
A new method for characterization of acid production by dairy starter cultures is presented. Microplates with integrated optical pH sensors are developed. Two fluorophores, a pH-sensitive and a pH-insensitive one are immobilised at the bottom of a polystyrene 96-well microtitre plate. The pH-insensitive fluorophore serves as an internal reference and makes calibration unnecessary. The sensor measures pH accurately in optically well-defined media. Particles and fluorophores contained in the bulk medium disturbed the measurements. Despite these disturbances it was possible to clearly sense differences in inoculum type and in inoculum sizes of cultures of Lactococcus lactis and of Streptococcus thermophilus at 30 and 37 degreesC. Besides a pH-related signal there is information about other changes during milk fermentation. The Cultivation results were compared with those from the established CINAC-method. From this comparison it can be concluded that the new method can be used reliably to characterize particularly a large number of strains for screening purposes but also for quality control.
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