Zusammenfassung
An improved portable detector for biological compounds, the chemiluminescence multichannel immunosensor (CL-MADAG), has been developed and characterised. The device is based on a capillary ELISA technique in combination with a miniaturised fluidics system and uses chemiluminescence as the detection principle. The fluidics system construction allows three chemiluminescence immunoassays to be ...
Zusammenfassung
An improved portable detector for biological compounds, the chemiluminescence multichannel immunosensor (CL-MADAG), has been developed and characterised. The device is based on a capillary ELISA technique in combination with a miniaturised fluidics system and uses chemiluminescence as the detection principle. The fluidics system construction allows three chemiluminescence immunoassays to be performed simultaneously within three fused silica capillaries (FSC). The CL-MADAG was characterised in a seizes of experiments with staphylococcal enterotoxin B (SEB) as a model toxin, the bacterial phage virus M 13 as a virus simulant, and a pathogenic strain of Escherichia coli as simulant for bacteria. It was shown that the CL-MADAG can assay liquid samples for these substances within 24 min. The detection limits were 5 ng/ml for SEB, 10(5) efu/ml for E. coli O157:H7 and 10(7) pfu/ml for M13. (C) 2002 Elsevier Science B.V All rights reserved.
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