Zusammenfassung
A new method is proposed for tracking fluorescing single molecules diffusing within a two-dimensional membrane. It is based on a confocal microscopy setup with a constantly rotating laser focus, which follows the position of the molecule. The optimization and efficiency of the method are theoretically studied for a broad range of experimentally realistic conditions. The proposed method allows for ...
Zusammenfassung
A new method is proposed for tracking fluorescing single molecules diffusing within a two-dimensional membrane. It is based on a confocal microscopy setup with a constantly rotating laser focus, which follows the position of the molecule. The optimization and efficiency of the method are theoretically studied for a broad range of experimentally realistic conditions. The proposed method allows for a long-time observation of diffusing molecules while allowing the application of fast spectroscopic techniques such as fluorescence decay time determination or fluorescence anisotropy measurements.
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