Zusammenfassung
The E1B-55K and E4orf6 oncoproteins of adenovirus type 5 are involved in the export of viral mRNAs, Previously, it cr as suggested that a complex composed of E1B-55K and E4orf6 serves as a nucleocytoplasmic transporter for viral mRNAs in which the E4orf6 protein directs both nuclear import and export, We now demonstrate that the E1B-55K protein itself shuttles efficiently in the absence of ...
Zusammenfassung
The E1B-55K and E4orf6 oncoproteins of adenovirus type 5 are involved in the export of viral mRNAs, Previously, it cr as suggested that a complex composed of E1B-55K and E4orf6 serves as a nucleocytoplasmic transporter for viral mRNAs in which the E4orf6 protein directs both nuclear import and export, We now demonstrate that the E1B-55K protein itself shuttles efficiently in the absence of E4orf6, In addition, E1B-55K trafficking was independent of the defined shuttle proteins Mdm2 or p53, which interacts with E1B-55K, The identified N-terminal E1B-55K leucine-rich nuclear-export signal (NES) conferred rapid nuclear export even in a heterologous system in contrast to the postulated E4orf6NES, Interestingly, although shuttling was blocked by inhibitors of the CRM1 mediated export pathway, E1B-55K inhibited neither the activity nor the trafficking of the retroviral shuttle proteins HIV-1 Rev and HTLV-1 Rex, In contrast, Rev or Rex blocked the nuclear export of E1B-55K, most likely by competing for essential export factors, Our results provide new insights into the regulation of the adenovirus mRNA export system and the processes of adenovirus mediated transformation.
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