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Electrophysiological and calcium-handling development during long-term culture of human-induced pluripotent stem cell-derived cardiomyocytes

Seibertz, Fitzwilliam ; Sutanto, Henry ; Dülk, Rebekka ; Pronto, Julius Ryan D. ; Springer, Robin ; Rapedius, Markus ; Liutkute, Aiste ; Ritter, Melanie ; Jung, Philipp ; Stelzer, Lea ; Hüsgen, Luisa M. ; Klopp, Marie ; Rubio, Tony ; Fakuade, Funsho E. ; Mason, Fleur E. ; Hartmann, Nico ; Pabel, Steffen ; Streckfuss-Bömeke, Katrin ; Cyganek, Lukas ; Sossalla, Samuel ; Heijman, Jordi ; Voigt, Niels



Zusammenfassung

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are increasingly used for personalised medicine and preclinical cardiotoxicity testing. Reports on hiPSC-CM commonly describe heterogenous functional readouts and underdeveloped or immature phenotypical properties. Cost-effective, fully defined monolayer culture is approaching mainstream adoption; however, the optimal age at ...

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