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A predicted CRISPR-mediated symbiosis between uncultivated archaea

Esser, Sarah P. ; Rahlff, Janina ; Zhao, Weishu ; Predl, Michael ; Plewka, Julia ; Sures, Katharina ; Wimmer, Franziska ; Lee, Janey ; Adam, Panagiotis S. ; McGonigle, Julia ; Turzynski, Victoria ; Banas, Indra ; Schwank, Katrin ; Krupovic, Mart ; Bornemann, Till L. V. ; Figueroa-Gonzalez, Perla Abigail ; Jarett, Jessica ; Rattei, Thomas ; Amano, Yuki ; Blaby, Ian K. ; Cheng, Jan-Fang ; Brazelton, William J. ; Beisel, Chase L. ; Woyke, Tanja ; Zhang, Ying ; Probst, Alexander J.



Abstract

CRISPR spacers in DPANN archaea target putative essential genes in their episymbionts and could be a widespread occurrence across diverse archaeal lineages. CRISPR-Cas systems defend prokaryotic cells from invasive DNA of viruses, plasmids and other mobile genetic elements. Here, we show using metagenomics, metatranscriptomics and single-cell genomics that CRISPR systems of widespread, ...

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