; Lamottke, Britta ; Tischer-Zimmermann, Sabine ; Schultze-Florey, Rebecca ; Goudeva, Lilia ; Heuft, Hans-Gert ; Arseniev, Lubomir ; Beier, Rita ; Beutel, Gernot
; Cario, Gunnar ; Fröhlich, Birgit ; Greil, Johann ; Hansmann, Leo
; Hasenkamp, Justin ; Höfs, Michaela ; Hundsdoerfer, Patrick ; Jost, Edgar ; Kafa, Kinan ; Kriege, Oliver ; Kröger, Nicolaus ; Mathas, Stephan ; Meisel, Roland ; Nathrath, Michaela ; Putkonen, Mervi ; Ravens, Sarina ; Reinhardt, Hans Christian ; Sala, Elisa ; Sauer, Martin G. ; Schmitt, Clemens ; Schroers, Roland ; Steckel, Nina Kristin ; Trappe, Ralf Ulrich
; Verbeek, Mareike ; Wolff, Daniel ; Blasczyk, Rainer ; Eiz-Vesper, Britta ; Maecker-Kolhoff, Britta | Dokumentenart: | Artikel | ||||
|---|---|---|---|---|---|
| Titel eines Journals oder einer Zeitschrift: | Journal of Clinical Investigation | ||||
| Verlag: | AMER SOC CLINICAL INVESTIGATION INC | ||||
| Ort der Veröffentlichung: | ANN ARBOR | ||||
| Band: | 133 | ||||
| Nummer des Zeitschriftenheftes oder des Kapitels: | 12 | ||||
| Datum: | 2023 | ||||
| Institutionen: | Medizin > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) | ||||
| Identifikationsnummer: |
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| Stichwörter / Keywords: | EPSTEIN-BARR-VIRUS; POSTTRANSPLANTATION LYMPHOPROLIFERATIVE DISEASE; RISK-FACTORS; RECIPIENTS; DISORDERS; INFECTIONS; IMMUNITY; THERAPY; PTLD; | ||||
| Dewey-Dezimal-Klassifikation: | 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin | ||||
| Status: | Veröffentlicht | ||||
| Begutachtet: | Ja, diese Version wurde begutachtet | ||||
| An der Universität Regensburg entstanden: | Ja | ||||
| Dokumenten-ID: | 76293 |
Zusammenfassung
BACKGROUND. Adoptive transfer of EBV-specific T cells can restore specific immunity in immunocompromised patients with EBV-associated complications.METHODS. We provide results of a personalized T cell manufacturing program evaluating donor, patient, T cell product, and outcome data. Patient-tailored clinical-grade EBV-specific cytotoxic T lymphocyte (EBV-CTL) products from stem cell donors ...

Zusammenfassung
BACKGROUND. Adoptive transfer of EBV-specific T cells can restore specific immunity in immunocompromised patients with EBV-associated complications.METHODS. We provide results of a personalized T cell manufacturing program evaluating donor, patient, T cell product, and outcome data. Patient-tailored clinical-grade EBV-specific cytotoxic T lymphocyte (EBV-CTL) products from stem cell donors (SCDs), related third-party donors (TPDs), or unrelated TPDs from the allogeneic T cell donor registry (alloCELL) at Hannover Medical School were manufactured by immunomagnetic selection using a CliniMACS Plus or Prodigy device and the EBV PepTivators EBNA-1 and Select. Consecutive manufacturing processes were evaluated, and patient outcome and side effects were retrieved by retrospective chart analysis.RESULTS. Forty clinical-grade EBV-CTL products from SCDs, related TPDs, or unrelated TPDs were generated for 37 patients with refractory EBV infections or EBV-associated malignancies with and without a history of transplantation, within 5 days (median) after donor identification. Thirty-four patients received 1-14 EBV-CTL products (fresh and cryopreserved). EBV-CTL transfer led to a complete response in 20 of 29 patients who were evaluated for clinical response. No infusion-related toxicity was reported. EBV-specific T cells in patients' blood were detectable in 16 of 18 monitored patients (89%) after transfer, and their presence correlated with clinical response.CONCLUSION. Personalized clinical-grade manufacture of EBV-CTL products via immunomagnetic selection from SCDs, related TPDs, or unrelated TPDs in a timely manner is feasible. Overall, EBV-CTLs were clinically effective and well tolerated. Our data suggest EBV-CTL transfer as a promising therapeutic approach for immunocompromised patients with refractory EBV-associated diseases beyond HSCT, as well as patients with preexisting organ dysfunction.TRIAL REGISTRATION. Not applicable.
Metadaten zuletzt geändert: 18 Mrz 2025 10:11
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