Boosting Reversible Photocontrol of a Photoxenase by an Engineered Conformational Shift

URN to cite this document:: urn:nbn:de:bvb:355-epub-782041
DOI to cite this document:: 10.5283/epub.78204

Mandl, Sabrina ; Sánchez, Janet ; Estévez, Miquel ; Bruckmann, Astrid

; Hiefinger, Caroline ; Osuna, Sílvia ; Hupfeld, Andrea

License: Creative Commons Attribution 4.0
PDF
Early View
(5MB)

Date of publication of this fulltext: 25 Nov 2025 06:28

Alternative links to fulltext:DOI Publisher

This publication is part of the DEAL contract with Wiley.

Details

Preview: Early View

Export bibliographical data

Abstract

Our study successfully explores strategies to effectively improve the photocontrol efficiency of light-sensitive enzymes, dubbed photoxenases, with photoswitchable unnatural amino acids (UAAs). The engineering of photoxenases is a versatile method for the reversible photocontrol in various applications. To boost the photocontrol of an established allosteric and heterodimeric photoxenase based on ...