| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Biomaterials | ||||
| Publisher: | ELSEVIER SCI LTD | ||||
| Place of Publication: | OXFORD | ||||
| Volume: | 26 | ||||
| Number of Issue or Book Chapter: | 33 | ||||
| Page Range: | pp. 6588-6598 | ||||
| Date: | November 2006 | ||||
| Institutions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth | ||||
| Identification Number: |
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| Keywords: | STEM-CELLS; TYROSINE KINASE; KIDNEY; CULTURE; DIFFERENTIATION; ALDOSTERONE; FAILURE; TUBULES; CLONING; artificial interstitium; polyester; kidney; tubules; stem cells; tissue engineering | ||||
| Dewey Decimal Classification: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 86 |
Web of ScienceAbstract
The construction of an artificial kidney module by tissue engineering or the application of cell-based therapies for the treatment of renal failure requires exact information regarding the cellbiological mechanisms of parenchyme development in combination with different kinds of biomaterials. To learn more about these processes tissue cultures are frequently used experimental tools. However, ...
Abstract
The construction of an artificial kidney module by tissue engineering or the application of cell-based therapies for the treatment of renal failure requires exact information regarding the cellbiological mechanisms of parenchyme development in combination with different kinds of biomaterials. To learn more about these processes tissue cultures are frequently used experimental tools. However, apart from experiments with early kidney anlagen there is a lack of suitable in-vitro models regarding the generation and long-term maintenance or renal tubules. In the present paper we like to demonstrate all advanced culture technique, which allows to generate tubular elements derived from renal stem cells. For the growth of tubules it is essential to fine-tune the interface between the embryonic tissue and the dead fluid space within a perfusion culture container by offering a polyester artificial interstitium. Culture was performed in IMDM supplemented with hormones and growth factors but using serum-free conditions over. 14 days, Formation of tissue was then analysed by immunohistochemistry and two-dimensional (2D) electrophoresis. Culture in pure IMDM leads to a complete loss of tissue formation. In contrast, application of aldosterone (A) induces the development of numerous polarised tubules. Surprisingly, addition of epidermal growth factor (EGF). a cocktail Of insulin transferrin and selenium (ITS). retinoic acid (RA), cholecalciferol (VitD(3)) or bovine pituitary extract (BPT) does not further improve development Of tubules, but leads to intensive cell Clustering and a decrease of tubule formation. 2D Western blots of developing tissue probed with soybean agglutinin (SBA) reveal a unique pattern of newly detected proteins. It is found that growth factors do not support hut abolish protein spots upregulated by aldosterone. It remains to be investigated, which cellbiological effect stimulates the embryonic cells to develop tubules in competition to cell clusters at the interphase of an artificial interstitium. (c) 2005 Elsevier Ltd. All rights reserved.
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